Daniel:Notebook/ComboLock/2017-1-31

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Revision as of 17:30, 31 January 2017 by >Djacobse (Created page with "=RCA Time Test (Started Friday)= Back to Calendar ==Epicentre RCA 12 hour (Started [[Daniel:Notebook/Co...")
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RCA Time Test (Started Friday)

Back to Calendar

Epicentre RCA 12 hour (Started Yesterday)

  1. qPCR
    1. Make 5.2X qPCR master mix according to following recipe
      1. 109.2 uL nfH2O
      2. 5.2 uL AmpF6.4
      3. 130 uL Kapa SYBR Fast
    2. Add 47 uL master mix to each well
    3. Add 2 uL sample and 1 uL AmpR indexed according to table
    4. qPCR Cycles
      1. 95C 3 min
      2. 95C 3 sec
      3. 55C 30 sec
      4. 72C 20 sec
      5. plate read
      6. goto b x24
      7. 72C 2 min
      8. 16C hold
  2. TBE Gel
    1. Mix 64 uL TBE, 16 uL 6x loading dye
    2. Aliquot 10 uL per sample/ladder lane onto parafilm
    3. Add 2 uL of sample or ladder to correct drop
    4. Load 10 uL in to well
    5. Run gel for 23 minutes at 230V
    6. Open gel and stain with 3 uL SYBR Gold for 3 minutes
    7. Rinse gel and image in gel doc