Daniel:Notebook/ComboLock/2017-2-10
Jump to navigation
Jump to search
Beads Binding Test (Started Yesterday)
Protocol-Part 2-qPCR
- qPCR
- Make qPCR master mixes according to following recipes
- Add 48 uL master mix to each well
- Add 2 uL sample according to table in following section
- qPCR Cycles
- 95C 3 min
- 95C 3 sec
- 55C 30 sec
- 72C 15 sec
- plate read
- goto b x24
- 72C 2 min
- 16C hold