Daniel:Notebook/ComboLock/2017-2-10

From ZhangLabWiki
Revision as of 00:57, 10 February 2017 by >Djacobse (→‎Protocol-Part 2-qPCR)
Jump to navigation Jump to search

Beads Binding Test (Started Yesterday)

Back to Calendar

Protocol-Part 2-qPCR

  1. qPCR
    1. Make qPCR master mixes according to following recipes
    2. Add 48 uL master mix to each well
    3. Add 2 uL sample according to table in following section
    4. qPCR Cycles
      1. 95C 3 min
      2. 95C 3 sec
      3. 55C 30 sec
      4. 72C 15 sec
      5. plate read
      6. goto b x24
      7. 72C 2 min
      8. 16C hold

    Plate Layouts

    File:20170209-Plate1-5.png

    File:20170209-Plate6-10.png