Daniel:Notebook/ComboLock/2017-2-10
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Beads Binding Test (Started Yesterday)
Protocol-Part 2-qPCR
- qPCR
- Make qPCR master mixes according to following recipes
- Add 48 uL master mix to each well
- Add 2 uL sample according to table in following section
- qPCR Cycles
- 95C 3 min
- 95C 3 sec
- 55C 30 sec
- 72C 15 sec
- plate read
- goto b x24
- 72C 2 min
- 16C hold
Master Mix | No X | Primer Pair | Forward Primer Amt (10 uM) | Reverse Primer Amt (10 uM) | uL nfH2O | uL 2X Kapa SYBR Fast | Total |
MMC1 | 73 | Primer24/Primer4RC | 73 | 73 | 1168 | 1460 | 2774 |
MMC2 | 2.2 | Primer6/Primer12RC | 2.2 | 2.2 | 35.2 | 44 | 83.6 |
MMT | 2.2 | PCCTAmpF/PCCT-AmpR | 2.2 | 2.2 | 35.2 | 44 | 83.6 |