Daniel:Notebook/ComboLock/2017-3-9

From ZhangLabWiki
Revision as of 00:58, 9 March 2017 by >Djacobse (Created page with "=Bolt Test (Started Yesterday)= Back to Calendar ==Protocol== ol start="3" <li>Bolt/Latch Hyb...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Bolt Test (Started Yesterday)

Back to Calendar

Protocol

ol start="3"

  • Bolt/Latch Hybridization
    1. Wash twice with 100 uL wash buffer
  • Elution
    1. Add 50 uL wash buffer to each sample
    2. Cook at 95C for 15 minutes
  • qPCR
    1. Make master mixes according to following recipes
    2.   Single Rxn MMC1 MMC2 MMP
      Primer Pair NA p24/p4RC p6/p12RC p24/p12RC
      uL Fwd Primer (10 uM) 1 10.2 10.2 10.2
      uL Rev Primer (10 uM) 1 10.2 10.2 10.2
      uL nfH2O 21 214.2 214.2 214.2
      uL Kapa SYBR Fast 25 255 255 255
      Total 48 489.6 489.6 489.6
    3. Add 48 uL master mix to each well
    4. Add 2 uL sample according to plate layout
    5. File:PlateLayout-20170309-BoltTest.png