Daniel:Notebook/ComboLock/2017-3-16
Jump to navigation
Jump to search
Circularization Comparison (T4 Poly/Ligase)
Protocol-Part 4
- Exonuclease Digestion
- Mix 15 uL Exo I (20U/uL) and 3 uL ExoIII (100U/uL)
- Add 2 uL to each sample, mix by swirling pipette tip
- Incubate for 1.5 hours at 37C
- Heat kill by incubating for 5 min at 95C
- Rolling Circle Amplification
- Samples will be aliquoted under following rules