Daniel:Notebook/ComboLock/2017-3-16

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Revision as of 18:23, 16 March 2017 by >Djacobse (Created page with "=Circularization Comparison (T4 Poly/Ligase)= Back to Calendar ==Protocol-Part 4== <ol start="7"> <li>Exonuclease Digestion</li> <ol type="A">...")
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Circularization Comparison (T4 Poly/Ligase)

Back to Calendar

Protocol-Part 4

  1. Exonuclease Digestion
    1. Mix 15 uL Exo I (20U/uL) and 3 uL ExoIII (100U/uL)
    2. Add 2 uL to each sample, mix by swirling pipette tip
    3. Incubate for 1.5 hours at 37C
    4. Heat kill by incubating for 5 min at 95C
  2. Rolling Circle Amplification
    1. Samples will be aliquoted under following rules