Daniel:Notebook/ComboLock/2017-3-25

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Revision as of 22:43, 25 March 2017 by >Djacobse (Created page with "=RCA Test= Back to Calendar This experiment is to test the effectiveness of RCA so that I can use the most efficient method of amplification fo...")
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RCA Test

Back to Calendar

This experiment is to test the effectiveness of RCA so that I can use the most efficient method of amplification for the cell-based experiments. This builds on the T4 experiments and the circular product production. I will be using that circular product to analyze the effectiveness of RCA.

Linear Product Production (Continued from yesterday)

  • TBE gel
  • Mix 64 uL TBE and 16 uL 6X dye
  • Aliquot 10 uL on to parafilm per sample/ladder
  • Add 2 uL reaction, 1 uL (10 uM) controls, and 1.5 uL ladder to appropriate aliquot
  • File:GelLanes-20170325-LinearProduct.png

  • Add 10 uL mix to gel lanes
  • Run gel for 24 minutes at 230V
  • Stain with 2 uL SYBR gold for 3 minutes
  • Rinse and image in gel doc
  • RCA Protocol