Daniel:Notebook/ComboLock/2017-3-29
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RCA Test (Started March 24)
Bglii Digestion
Testing bglii digestion of RCA product. No qPCR to start with (RCA should be enough)
Reagent | Single Rxn Vol | Master Mix (30.2X) |
RCA Rxn | 2 | 0 |
Bglii Cutter Primer + (100 uM) | 2 | 60.4 |
10X Buffer 3.1 | 2 | 60.4 |
Bglii | 1 | 0 |
nfH2O | 13 | 392.6 |
Total | 20 | 513.4 |
- 5 min 95C
- Ramp to 50C at 0.2C/s
- 10 min 50C
- 2 hr 37C; When this cycle starts add 1 uL Bglii
- Heat kill with 65C for 20 min