Daniel:Notebook/PosSequencing/2017-4-12
Jump to navigation
Jump to search
==
Bead Loading Test
- Oil phase
- Start from yesterday's oil prep (Span80-4.5%, Tween20-0.1%, TritonX100-0.05% in mineral oil)
- Oil/aqueous mixing (adopted from Shao/Church-adjusted for Church protocol bead concentration)
- Mix 95 uL TE buffer and 10 uL magnetic Dynabeads; vortex to mix
- Mix 20 uL aqueous into 200 uL oil mixture
- Vortex for 30 seconds
- Repeat mixing to 100 uL (5 times)
- Mix with vortexer for 3 minutes
- Dilute with pure mineral oil for imaging (1:100)