Daniel:Notebook/PosSequencing/2017-4-12

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Revision as of 20:03, 12 April 2017 by >Djacobse
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Emulsion Test-Variables (Started Yesterday)

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Bead Loading Test

  1. Oil phase
    1. Start from yesterday's oil prep (Span80-4.5%, Tween20-0.1%, TritonX100-0.05% in mineral oil)
  2. Oil/aqueous mixing (adopted from Shao/Church-adjusted for Church protocol bead concentration)
    1. Mix 95 uL TE buffer and 10 uL magnetic Dynabeads; vortex to mix
    2. Mix 20 uL aqueous into 200 uL oil mixture
    3. Vortex for 30 seconds
    4. Repeat mixing to 100 uL (5 times)
    5. Mix with vortexer for 3 minutes
    6. Dilute with pure mineral oil for imaging (1:100)