Daniel:Notebook/ComboLock/2017-4-18
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Circularization Test (Started Jan 23, 2017)
Sequencing Results
These just show another poorly hybridized product. The aligned bam files can be found in genome miner at:
/media/NAS3_volume2/Dan/combolock/miseq_20170414
Most of the reads have ~50bp matching, which is almost always the AmpF signal. I also discovered something upon close examination, shown in the read below. There is a small overlap at the final bases of LinkerR and first few bases of primer24RC. Apparently at high enough concentrations this causes trouble.
M00159:97:000000000-B4K7F:1:1101:11875:18466 | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG | ACTGTATTGTA | TTTTTTTTTTTT | AATGATACGGCGACCACCGA | GATCTACACCACTCTCAGAT | GTTATCGAGGTCCGACGATGTCAGAC | CGGGCCCACGGG |