Matt:LabNotes/2017-4-22

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Revision as of 02:25, 23 April 2017 by >Mzcai (Created page with "=In tube SplintR Test with formamide and ET SSB= ==Test Conditions== #Standard: SplintR only #SplintR incubated for 30min instead of 15min #SplintR + 10% formamide #SplintR +...")
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In tube SplintR Test with formamide and ET SSB

Test Conditions

  1. Standard: SplintR only
  2. SplintR incubated for 30min instead of 15min
  3. SplintR + 10% formamide
  4. SplintR + 20% formamide
  5. SplintR + 30% formamide
  6. SplintR + 250ng ET SSB
  7. SplintR + 250ng ET SSB added first
  8. Positive Control: Ampligase

Padlock Probes

  • Same as Matt:LabNotes/2017-4-7 with the addition of ppMALAT1 for consistency
  • ppCUX2
  • ppBCL11B
  • ppRELN_1
  • ppGFAP
  • ppMALAT1
    • Has the MALAT1_template

Protocol

  1. Add 100nM of each padlock probe to hybridization mix containing SplintR buffer + 0%, 10%, 20% or 30% formamide l
  2. Hybridize at 55C for 13 hours