Matt:LabNotes/2017-4-22
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In tube SplintR Test with formamide and ET SSB
Test Conditions
- Standard: SplintR only
- SplintR incubated for 30min instead of 15min
- SplintR + 10% formamide
- SplintR + 20% formamide
- SplintR + 30% formamide
- SplintR + 250ng ET SSB
- SplintR + 250ng ET SSB added first
- Positive Control: Ampligase
Padlock Probes
- Same as Matt:LabNotes/2017-4-7 with the addition of ppMALAT1 for consistency
- ppCUX2
- ppBCL11B
- ppRELN_1
- ppGFAP
- ppMALAT1
- Has the MALAT1_template
Protocol
- Add 100nM of each padlock probe to hybridization mix containing SplintR buffer + 0%, 10%, 20% or 30% formamide l
- Hybridize at 55C for 13 hours