Daniel:Protocols/Qubit

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Qubit Quantification[edit]

Back to Main

Protocol[edit]

  • Note: choose appropriate Qubit buffer and dye for samples (ssDNA, RNA, dsDNA)
  1. Mix 199 uL Qubit buffer with 1 uL dye per sample +2 for standards
  2. For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
  3. For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
  4. Let sit in the dark 5 minutes
  5. Measure in Qubit using new standards