Daniel:Notebook/PosSequencing/2017-5-11

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Revision as of 18:59, 11 May 2017 by >Djacobse (Created page with "=Bead Binding Test 2 (Started Yesterday)= Back to Calendar ==Image Processing== <ol> <li>Open ...")
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Bead Binding Test 2 (Started Yesterday)

Back to Calendar

Image Processing

  1. Open new image in photoshop; use resolution 1344x1024 (same as .jpg images from microscope)
  2. Import brightfield and DAPI images to photoshop file
  3. Adjust levels as deemed useful using Image->Adjustments->Levels
  4. Create new red solid color layer
  5. Click on the mask icon (image next to red) and then go to Image->Apply Image...
  6. Use DAPI image as "Source" and choose "Multiply" under "Blending"
  7. Hide the original DAPI layer and save blended image

Results

<gallery perrow=2 heights=300px widths=300px caption="DAPI overlays for Sample 1 prior to melting"> File:|Field File:Sample1-dsDNA-Overlay-F2-Blend.png|Field 2 <gallery>

<gallery perrow=3 heights=300px widths=300px caption="DAPI overlays for Sample 2 prior to melting"> File:Sample2-dsDNA-Overlay-F1-BlendAdj.png|Field 1 File:Sample2-dsDNA-Overlay-F2-BlendAdj.png|Field 2 File:|Field 3 <gallery>