Daniel:Notebook/ComboLock/2017-5-16

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Revision as of 22:45, 16 May 2017 by >Djacobse (→‎Base Complexities)
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Production Run (Started Friday 4/28; Libary Prep)

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Sequencing Results

These results are from the sequencing run performed 5/8/2017.

Read Statistics

Sample Condition Reads Aligned Reads Unaligned Pct Aligned Missing UMIs (%) Poisson Lambda Poissonian E[0] (%) UMI Missing Enrichment
1X NEB Sample 254728 21861 92.1% 34.17 3.89 2.0 16.7
5AX NEB Positive Control 119220 10206 92.1% 31.2 1.82 16.2 1.9
1Y TF Sample 197959 23195 89.5% 42.53 3.02 4.9 8.7
5AY TF Positive Control 63074 6955 90.1% 51.37 0.96 38.3 1.3
4AY TF Dephosphorylated 133660 15412 89.7% 72.47 2.03 13.1 5.5
NA Randomly Generated 250,000 UMIs     2.26 3.81 2.21 1.0

UMI Counting

Base Complexities

Discussion

Sequencing results are good overall, at least good enough to move on to the BSA positive control and possibly cells for mRNA.