Daniel:Notebook/PosSequencing/2017-5-17
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Bead Binding Test 3
Trying to test the bead binding and denaturation on the beads again. This time we'll use high pH as the denaturant. From the paper above it says the streptavidin bond is stable to about pH 11, and according to this website DNA denaturation occurs at pH>10. I will use the alkaline lysis solution from Andrew's notes
ALS Buffer
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA
NS buffer
- 3 mL H20
- 3 mL 2M Tris-HCl
- 4 mL 1N HCl