Daniel:Notebook/ComboLock/2017-6-7
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Sensitivity and Specificity Experiments (Started Monday)
Protocol
- qPCR
- Make 17.2X master mix according to following (1X)
- 361.2 uL nfH2O (21)
- 17.2 uL 10 uM AmpF6.4Sol (1)
- 430 uL 2X Kapa SYBR Fast Master Mix (25)
- Add 47 uL master mix to each well
- Add 2 uL sample and 1 uL AmpR Index (10 uM) according to plate layout File:PlateLayout-SensSpec-20170607.png
- qPCR Cycles
- 95C 3 min
- 95C 3 sec
- 55C 30 sec
- 72C 20 sec
- plate read
- goto b x30
- 72C 2 min
- 16C hold