Daniel:Notebook/ComboLock/2017-6-7

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Sensitivity and Specificity Experiments (Started Monday)

Back to Calendar

Protocol

  1. qPCR
    1. Make 17.2X master mix according to following (1X)
      1. 361.2 uL nfH2O (21)
      2. 17.2 uL 10 uM AmpF6.4Sol (1)
      3. 430 uL 2X Kapa SYBR Fast Master Mix (25)
    2. Add 47 uL master mix to each well
    3. Add 2 uL sample and 1 uL AmpR Index (10 uM) according to plate layout
    4. File:PlateLayout-SensSpec-20170607.png
    5. qPCR Cycles
      1. 95C 3 min
      2. 95C 3 sec
      3. 55C 30 sec
      4. 72C 20 sec
      5. plate read
      6. goto b x30
      7. 72C 2 min
      8. 16C hold