Daniel:Notebook/PosSequencing/2017-7-12
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emPCR8 (Started Thursday July 6)
- Dye hybridization
- Mix 20 uL ssDNA (lambda digested) and 20 uL dsDNA (original, non-digested template)
- Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
- Incubate in the dark (RT) for 15 minutes
- Magnet pulldown, remove supernatant and add 40 uL 2X SSC buffer
- Add 15 uL to a slide for analysis
- Seal coverslip with nail polish and wait approximately 30 minutes to dry