Daniel:Notebook/PosSequencing/2017-7-14

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emPCR 9-Concentration Comparison (Started Wednesday)

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Protocol-Part 3

  1. Dye hybridization
    1. Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
    2. Incubate in the dark at 75C for 5 minutes
    3. Incubate at 55C for 30 minutes
    4. Incubate in the dark (RT) for 15 minutes
    5. Magnet pull down, remove supernatant and add 40 uL 2X SSC buffer
    6. Add 15 uL to a slide for analysis
    7. Seal coverslip with nail polish and wait approximately 30 minutes to dry