Daniel:Notebook/PosSequencing/2017-7-14
Jump to navigation
Jump to search
emPCR 9-Concentration Comparison (Started Wednesday)
Protocol-Part 3
- Dye hybridization
- Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
- Incubate in the dark at 75C for 5 minutes
- Incubate at 55C for 30 minutes
- Incubate in the dark (RT) for 15 minutes
- Magnet pull down, remove supernatant and add 40 uL 2X SSC buffer
- Add 15 uL to a slide for analysis
- Seal coverslip with nail polish and wait approximately 30 minutes to dry