Alice:LabNotes/2007-12-12

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Revision as of 07:44, 26 December 2007 by >Zsakura2
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Agarose gel on post-PCR product of PGP1Ver3 Primers

' well 1 well 2-13
Low Mass bp Ladder 3 ul 0 ul 0 ul 0 ul 0 ul 0 ul 0 ul 0 ul
Novex 5x TBE loading dye 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul 2 ul
post-PCR sample 0 ul 3 ul 3 ul 3 ul 3 ul 3 ul 3 ul 3 ul


File:ZhangLab 2 2007-12-12 13hr 32min.jpg Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice.