Alice:LabNotes/2007-12-12
Jump to navigation
Jump to search
Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | |
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
There were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice.
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected.