Kun:LabNotes/ASE/2007-12-16

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Revision as of 00:03, 17 December 2007 by KunZhang (talk | contribs) (New page: ==Exp.1 Exon capture experiment== NA10835(353ng/ul) 3ul E55kv1 probes 107nM 17ul 10X Ampligase buffer 2.25ul 95C 10min -> 60C 40h -> add 2.5ul NSL mix -> 60C 1h ->...)
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Exp.1 Exon capture experiment

   NA10835(353ng/ul)        3ul
   E55kv1 probes 107nM     17ul
   10X Ampligase buffer  2.25ul

95C 10min -> 60C 40h -> add 2.5ul NSL mix -> 60C 1h -> (95C 2min -> 60C 1h) x 20 -> 60C xh -> 37C 1min -> add 2ul exo I&III -> 37C 2h -> 95C 2min -> 4c hold