Alice:LabNotes/2009-5-29
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PCR with AmpF/R6.3Sol for end sequencing (done by Ida)
detailed information on the templates can be found here:[[1]] b - exome probe sets captured with PGP2 (0.25ng/ul) c - exome probe sets captured with PGP3 (0.26ng/ul) x2 Template (b/c) 1ul 2X iProof Mastermix 50ul 100ul AmpF6.3SoL (10uM) 4ul 8ul AmpR6.3SoL (10uM) 4ul 8ul 50X SYBG I 0.8ul 1.6ul H2O 41.2ul 82.4ul 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 4 -> (98C 10S -> 72C 20S) x 5 -> 72C 3 min -> 15C hold.
TBE gel result: File:ZhangLab 2 2009-05-29 11hr 43min.jpg