AlanFung:Protocol/FACScan

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Revision as of 01:50, 18 June 2009 by >Alan6017518 (→‎Cytometer Setup)
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FACScan (Becton Dickinson)

Cytometer Appearance (Bottom Panel)

-------------   --------------  --------------
| Sheath    |   |.2um Sheath |  |   Waste    |
| Tank      |   |Filter      |  |   Tank     |
-------------   --------------  --------------

Cytometer Setup

  • Turn on both the cytometer and the computer
  • Open up the bottom panel on the cytometer
  • Make sure the system is pressurized
(Vent valve should be in the upwards position)
(Sheath tank should be tight and non-movable)
(No gap between the sheath tank and the top of the rack)

Purge bubbles trapped in the filter

  • Pull white cap off from the tube coming out from the sheath filter