05/05/2015 Library Order

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Library Info file: File:05-05-2015 library info v4.xlsx

Library guides: File:05-05-2015 library order v4.xlsx

Notes: This library order contains 4 libraries to be used in the CRISPR screen and single-cell sequencing project: epigenetic modifier library, pluripotency gene library, lineage specific gene library, mediator complex library. For each library, we included a library for both the m scaffold and the f scaffold. The m scaffold allows for a polyA sequence to be cloned in near the end, next to a barcode that we will create with a random primer. To de-multiplex the barcode after sequencing, we will sequence the CRISPR vectors to link spacer sequences to barcodes. For the f scaffold, there is a polyA sequence closer to the spacer sequence. There are also the full chr 21 haplotype libraries (2 mismatches away) to be used in the Genome-wide allele screen and chromosome knockout project. These libraries have a modified scaffold that allows for cloning in of a barcode that we can hybridize RNA-FISH probes to.