AlanFung:LabNotes/CTCF/2010-3-22

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PAGE gel Quantification of spacer DNA[edit]

File:ZhangLab 2 2010-03-22 10hr 37min.jpg

  • Gel quantification suggests that I did in fact over-estimated the amount of DNA used, I used 3.8ng as a starting amount.

Repeat experiment with new parameters[edit]

Sample
1)85ul of 0.38ng/ul spacer DNA
2)5, 10, 50, 100ng of PCr product from Sam
3)-ve control

Improvements
*double amount of adapters
*Increase ligation incubation time