AlanFung:LabNotes/CTCF/2010-3-22
Jump to navigation
Jump to search
PAGE gel Quantification of spacer DNA[edit]
File:ZhangLab 2 2010-03-22 10hr 37min.jpg
- Gel quantification suggests that I did in fact over-estimated the amount of DNA used, I used 3.8ng as a starting amount.
Repeat experiment with new parameters[edit]
Sample 1)85ul of 0.38ng/ul spacer DNA 2)5, 10, 50, 100ng of PCr product from Sam 3)-ve control Improvements *double amount of adapters *Increase ligation incubation time