AlanFung:LabNotes/CTCF/2010-4-8

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Denaturing Gel Quantification[edit]

  • 0.5ul low mass ladder + 4.5ul ddh2o + 5ul 2x TBU Buffer
  • 2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer

File:ZhangLab 2 2010-04-08 12hr 46min.jpg

  • By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. It appears that we still have some half ligated and non ligated product.