AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31

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PCR Amplification of Captured product[edit]

NA12878 NA18507 NA20431 Jurkat New Jurkat Old[edit]

1 well (uL) X 6
Template 10
2X iProof Mastermix 50 300
Amp F6.3 Sol (100uM) 0.4 2.4
Amp R6.3Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8
H2O 31.2 187.2
Total Volume 90 540

98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

TBE Gel[edit]

File:ZhangLab 2 2010-01-04 12hr 26min.jpg