AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31
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PCR Amplification of Captured product[edit]
NA12878 NA18507 NA20431 Jurkat New Jurkat Old[edit]
1 well (uL) | X 6 | |
Template | 10 | |
2X iProof Mastermix | 50 | 300 |
Amp F6.3 Sol (100uM) | 0.4 | 2.4 |
Amp R6.3Sol (100uM) | 0.4 | 2.4 |
50X SYBG I | 0.8 | 4.8 |
H2O | 31.2 | 187.2 |
Total Volume | 90 | 540 |
98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold