AlanFung:LabNotes/Capturing/CPG97K Low-input/2009-11-25

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11/12/09[edit]

1well (ul) X 8 X1.1
Template 10
2X Phusion 50 400 440
AmpF6.2SoL (10uM) 4 32 35.2
AmpR6.2Sol (10uM) 4 32 35.2
50X SYBG I 0.8 6.4 7.04
H2O 31.2 249.6 274.56

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold.