AlanFung:LabNotes/Cell/2009-10-10

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MEF[edit]

  • Make 9x10cm MEF plates for MCM

HUES9[edit]

Split (1:8)from 1x10cm to 1 well (MEF) and 1x10cm (Matrigel)

H9[edit]

Split (1:6) form 1x6well to 1x10cm (Matrigel) and (1:6) 1x6well to 3x6wells

HUES53[edit]

  • Check and Feed, Images below
  • Split 1:2 10cm matrigel dishes when confluent, then collect the day after.

MEF[edit]

Matrigel[edit]

  • HUES 53 appears to form more elongated colonies with sharper edges, no signs of differentiation.
  • 1:5