AlanFung:LabNotes/EZ/2009-8-14

From ZhangLabWiki
Jump to navigation Jump to search

EZ DNA Methylation Direct - GM20431 cells[edit]

Objective[edit]

  • Extract DNA from 2000 GM20431 cells
  • Elute DNA with 8uL elution buffer
  • Use all 8uL dna as template for one pair of primer
  • Quantify amount of DNA with Nanodrop Qubit and Gel quantification
  • Perform serial dilution to obtain
200ng methylated DNA
100ng methylated DNA
50ng methylated DNA
30ng methylated DNA
10ng methylated DNA
' 2000 cells 100,000 cells
M-Digestion Buffer 10ul 13ul
Sample up to 9ul up to 12ul
Proteinase K 1ul 1ul
H20 X ul x ul
Total Volume 20ul 26 ul