AlanFung:LabNotes/EZ/2009-8-14
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EZ DNA Methylation Direct - GM20431 cells[edit]
Objective[edit]
- Extract DNA from 2000 GM20431 cells
- Elute DNA with 8uL elution buffer
- Use all 8uL dna as template for one pair of primer
- Quantify amount of DNA with Nanodrop Qubit and Gel quantification
- Perform serial dilution to obtain
200ng methylated DNA
|
100ng methylated DNA
|
50ng methylated DNA
|
30ng methylated DNA
|
10ng methylated DNA
|
|
'
|
2000 cells
|
100,000 cells
|
M-Digestion Buffer |
10ul |
13ul
|
Sample |
up to 9ul |
up to 12ul
|
Proteinase K |
1ul |
1ul
|
H20 |
X ul |
x ul
|
Total Volume |
20ul |
26 ul
|
|