AlanFung:LabNotes/Probe/2012-5-7

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Troubleshooting May7[edit]

  • Noi and I think that the probes are not digested completely

USER Digestion[edit]

  • from the 75.5ng/ul tube, take 40ul product add 2.5ul USER enzyme
  • Incubate at 37C for 1.5hr and run a denaturing gel
  • Since I ran a TBE gel on my end product from last week, I will rerun on a denaturing gel so that the 2nd structure wont be the reason why we see the larger than expected DNA size

File:ZhangLab 2 2012-05-07 13hr 31min.jpg

  • Turns out the end product is at the expected size, it was the TBE gel leading to larger than expected DNA size.
  • Will move on with the tube we started today and pool it with the end product and perform a size selection for both tubes.