AlanFung:LabNotes/Sequencing/2009-11-18
Jump to navigation
Jump to search
Re-amplification of PhiX control library[edit]
PCR:
*16 | *1.1 | ||
PhiX Control DNA (10nM) | 0.025 | 0.400 | |
Syb_FP5 (100uM) | 0.200 | 3.200 | 3.520 |
Syb_RP7 (100uM) | 0.200 | 3.200 | 3.520 |
2X Phusion | 50.000 | 800.000 | 880.000 |
50X SYBR Green I | 0.200 | 3.200 | 3.520 |
H20 | 50.000 | 800.000 | 880.000 |
98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 5 cycles -> 72C 3min -> 15C
Qiaquick column purification X4 and elute in 30ul of 0.1% Tween 20-EB.
Nanodrop result: 14.8ng/ul
PAGE gel result: QPCR result: