AlanFung:LabNotes/Sequencing/2009-11-27
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- Ethanol Precipitation
- Dissolve all in 14ul ddH2O
QPCR Quantification[edit]
Setup Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10)
X8 | X1.1 | ||
DNA | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 1.600 | 1.760 |
Syb_RP7 (100uM) | 0.200 | 1.600 | 1.760 |
2X Phusion | 25.000 | 200.000 | 220.000 |
50X SYBR Green I | 0.200 | 1.600 | 1.760 |
dH2O | 24.000 | 192.000 | 211.200 |
- Dilute sample, 1ul in 9ul ddh2o
- mix 2ul 1/10 sample with 98ul mix
- aliquot 24ul to each well
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Sum | Ave | Diff | 2^ | X10 | ||
CVF-N-S | 8.162 | 24.091 | 8.030 | 2.819 | 7.057 | 70.567 |
0.070 | 8.040 | |||||
7.889 | ||||||
PhiX Stock | 11.020 | 32.548 | 10.849 | |||
0.070 | 10.883 | |||||
10.645 | ||||||
CVF-N A/B | 7.850 | 32.161 | 8.040 | 1.791 | 3.460 | 34.599 |
0.050 | 8.002 | |||||
8.146 | ||||||
8.163 | ||||||
PhiX Stock | 9.665 | 29.493 | 9.831 | |||
0.050 | 9.876 | |||||
9.952 | ||||||