AlanFung:Protocol/MetaMorph Image Analysis

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Guide[edit]

File:MetaMorph Basic Commands.pdf File:MetaMorph Dropin Commands.pdf

Count Nuclei (P.789 in Dropin Commands)[edit]

  • Use this application module to segment images that are used to identify and differentiate cell nuclei.
  • The segmentation labels each isolated and identified nucleus as a different color index to enable you to see a visual separation between nuclei that are close or touching.
  • Source images must be 16-bit images.

Three settings are available to you:

  • Approximate minimum nuclei width, Approximate maximum nuclei width, and the minimum gray level intensity of the nuclei above the local background.
  • Any nuclei-like pattern in the image less than the specified minimum width will be considered to be noise, and will be excluded from the segmentation.

Parameters[edit]

Determine Dimensions[edit]

Determine Intensity above local background[edit]

Example[edit]

  • Original Image
  • Segmentation Image
  • Parameters
    • Approximate min width: 5um
    • Approximate max width: 30um
    • Intensity above local background: 5000graylevels
  • Results