AlanFung:Protocol/MetaMorph Image Analysis
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Guide[edit]
File:MetaMorph Basic Commands.pdf File:MetaMorph Dropin Commands.pdf
Count Nuclei (P.789 in Dropin Commands)[edit]
- Use this application module to segment images that are used to identify and differentiate cell nuclei.
- The segmentation labels each isolated and identified nucleus as a different color index to enable you to see a visual separation between nuclei that are close or touching.
- Source images must be 16-bit images.
Three settings are available to you:
- Approximate minimum nuclei width, Approximate maximum nuclei width, and the minimum gray level intensity of the nuclei above the local background.
- Any nuclei-like pattern in the image less than the specified minimum width will be considered to be noise, and will be excluded from the segmentation.
Parameters[edit]
Determine Dimensions[edit]
Determine Intensity above local background[edit]
Example[edit]
- Original Image
- Segmentation Image
- Parameters
- Approximate min width: 5um
- Approximate max width: 30um
- Intensity above local background: 5000graylevels
- Results