AlanFung:Protocol/Qubit
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Qubit Fluorometer Protocol[edit]
Samples & Materials[edit]
- Qubit fluorometer Invitrogen Cat. Q32857
- Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830)
- Quant-IT DSDNA BR Assay Kit Invitrogen Cat. Q32850
- Quant-IT DSDNA HS Assay Kit Invitrogen Cat. Q32851
- Quant-IT RNA Assay Kit Invitrogen Cat. Q32852
- Quant-IT Protein Assay Kit Invitrogen Cat. Q33211
File:Qubit1.jpg File:Qubit2.jpg
Calibrating the Qubit Fluorometer[edit]
Running a New Calibration[edit]
- Insert standard #1
- Press <Go>
- Wait for ~5s
- Insert standard #2
- Press <Go>
Using the Last Calibration[edit]
- Highlight <Use Last Calibration>
- Press <Go>
Reading Samples[edit]
- Insert sample
- Press <Go>
- Read the result on the display
- Record the reading
- Remove the sample form the instrument
- Insert the next sample
- Press <Go>
- Repeat until all samples have been read
Calculation the Concentration of your Sample[edit]
- Qubuit fluorometer gives values in either ug/ml or ng/ml
- Concentration of your sample = QF value x (200/x)
*where QF = the value given by the Qubit fluorometer x = the number of microliters of sample you added to the assay tube
- This equation generates a result with the same units as the value given by the Qubit fluorometer