Alice:LabNotes/2007-11-11
14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15.
Sample | Sequencing result | |
1 | no | |
2 | no | |
3 | no | |
4 | yes | |
5 | yes | |
6 | yes | |
7 | yes | |
8 | yes | |
9 | yes | |
10 | yes | |
11 | yes | |
12 | no | |
13 | yes | |
14 | no | |
15 | yes | |
16 | no | |
Sequencing Results:
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows:
SNP: rs1264899
Primer: rs1264899_cF/rs1264899_cR
Primer location: Hues6Ver2c F1/F4
Sample #13:
Template: PGP1 gDNA
File:SNP.png
Sample #15:
Template: GC1F cDNA
File:SNP2.png