Alice:LabNotes/2007-12-12
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Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | |
Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times.
Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected.