Alice:LabNotes/2008-1-22
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Experiment 1: Padlock preparation continued
Step 10: Precipitation
1. Add 10% of sodium acetate and 2.5x 100% ethanol.
2. Add 1uL Glycoblue so that one can precipitate better and see the DNA.
3. Freeze in -70C for 20 min, spin at 14,000 rpm for 15 min at 4C, discard supernatant with pipette.
4. Add 500 uL of 75% ethanol to wash DNA, spin at 8000 rpm for 5 min at 4C, discard supernatant with first large tip and then small tip to remove as much liquid as possible.
5. Let dry in hood ~ 10 min.
6. Add 40 uL of water to the tube to resolve the DNA in, leave at least 15 min.