Alice:LabNotes/2008-5-8
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PCR on 27k probe (15ng)[edit]
reaction system x2 H2O 32.1ul 64.2ul 10x JumpStartTaq Buffer 5ul 10ul MgCl2(25mM) 3ul 6ul dNTP(10mM) 2ul 4ul AmpF6.2/R6.2 (10uM) 2ul 4ul 50x SYBG I 0.4ul 0.8ul JumpStartTaq 0.5ul 1ul Products of Circluarization 5ul 10ul Total 50ul 100ul
94C 3min -> 20 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 30 sec -> 72 30 sec) -> 72C 3min -> 4C hold