Alice:LabNotes/2008-7-9

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Experiment 1: Optimization of the padlock captureing efficiency[edit]

PCR:

Real time PCR reaction system:
                                                               
H2O                                                21.4ul      
10X iTaq buffer                                       5ul        
MgCl2(25mM)                                           6ul        
50x SYBG I                                          0.4ul       
dNTP(2.5mM)                                           8ul        
primer AmpF 6.2 Sol(10uM)                             2ul
primer AmpR 6.2 Sol(10uM)                             2ul        
template(cosmic 3760 probe)                           5ul        
Platium Taq polymerase                              0.2ul
Total                                                50ul
Results:
File:7-9-08 PCR.jpg
The red curve on top is the product of cosmic 3760 probe after circularization, and the green curve is the control group with
cosmic probe obtained from Ida.

File:ZhangLab 2 2008-07-09 18hr 25min.jpg
Note:10 bp ladder should not be used whereas the product after circularization should be around 300-400 bp,
so 100bp ladder should be used instead to show better results.