Alice:LabNotes/2008-7-9
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Experiment 1: Optimization of the padlock captureing efficiency[edit]
PCR:
Real time PCR reaction system: H2O 21.4ul 10X iTaq buffer 5ul MgCl2(25mM) 6ul 50x SYBG I 0.4ul dNTP(2.5mM) 8ul primer AmpF 6.2 Sol(10uM) 2ul primer AmpR 6.2 Sol(10uM) 2ul template(cosmic 3760 probe) 5ul Platium Taq polymerase 0.2ul Total 50ul
Results: File:7-9-08 PCR.jpg The red curve on top is the product of cosmic 3760 probe after circularization, and the green curve is the control group with cosmic probe obtained from Ida. File:ZhangLab 2 2008-07-09 18hr 25min.jpg Note:10 bp ladder should not be used whereas the product after circularization should be around 300-400 bp, so 100bp ladder should be used instead to show better results.