Alice:LabNotes/2009-7-6

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The detailed capture reaction setup can be found under LabNotes 6/28/09
Pooled all the captured product based on the following table:
sample a1 a2 a3 a4 ' b1 b2 b3 b4
concentration (ng/ul) 16 9.7 6.7 12.3 9.4 7.6 5.1 9.8
volume (ul) 10 8.2 17 9.5 15 9.3 20 10.5
total (ng) 160 80 115.2 116.8 141 70.5 101.5 103
sample c1 c2 c3 c4 ' d1 d2 d3 d4
concentration (ng/ul) 13 7.2 4.9 5.4 14 7.8 6.6 7.4
volume (ul) 10 9 19 17.5 10 9 15 14
total (ng) 130 65 93.6 95 140 70 100.8 102.2
sample e1 e2 e3 e4 ' f1 f2 f3 f4
concentration (ng/ul) 11 3.1 3.4 4.7 9.5 5.5 2.2 4.6
volume (ul) 7.3 13 17 12.5 2.9 2.5 9 4.4
total (ng) 80.3 40.15 57.8 58.6 27.5 13.8 19.8 20
sample DF1 DF2 DF3 DF4 ' FS1 FS2 FS3 FS4
concentration (ng/ul) 17.7 10.1 6.2 11.7 15.3 4.1 5.4 5.2
volume (ul) 9.2 8.1 19 10.2 8.6 16 18 18.5
total (ng) 163.6 81.8 117.8 119.4 131.8 65.9 95 111.7
perform E-gel size selection after pooling all the samples together (8 total):
assume 70% recovery rate, the total amount of DNA in each sample set is:
PGP1_070809: 25ul (330.4ng)
PGP2_070809: 30ul (291ng)
PGP3_070809: 32ul (269ng)
NA12878_070809: 26ul (289ng)
PGP5_070809: 31ul (166ng)
PGP7_070809: 17ul (65ng)
DF6-9-9_070809: 33ul (177ng)
foreskin_070809: 44ul (283ng)
Since PGP7 had very little amount of DNA, it was not send out for shearing. And I will need to repeat the capture on sample f3.
Sent out the following tubes for Covaris shearing to Dr. Billy Li (Harvard) on 7/8/09
PGP1_070809: 25ul (13ng/ul)
PGP2_070809: 30ul (9.7ng/ul)
PGP3_070809: 32ul (8.4ng/ul)
NA12878_070809: 26ul (11ng/ul)
PGP5_070809: 31ul (5.5ng/ul)
DF6-9-9_070809: 33ul (5.4ng/ul)
foreskin_070809: 44ul (6.4ng/ul)