Alice:LabNotes/2009-9-7
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Re-amplification of PhiX control library[edit]
PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul 0.8ul Syb_RP7(100uM) 0.2ul 0.8ul 2x phusion master mix 50ul 200ul 50x SYBR geen 0.2ul 0.8ul H2O 50ul 200ul 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 5 cycles -> 72C 3min -> 15C
Qiaquick column purification and elute in 30ul of EB. PAGE gel result: File:ZhangLab 2 2009-09-09 16hr 02minphix.jpg QPCR result: 1.59nM