Andrew:Notebook/AR150618 C1
31 nL MDA on C1 with GM12878[edit]
Overview[edit]
Motivation[edit]
Reagents[edit]
Protocol[edit]
File:Protocol Worksheet AR150618 C1 v1-1.png
File:Protocol Worksheet AR150618 C1 v1-2.png
File:Protocol Worksheet AR150618 C1 v1-3.png
File:AR150618 thermalProfile.png
Results[edit]
Cell capture[edit]
Initials | Date (YYMMDD) | Chamber | Well | i7 | i5 | i7 sequence (RC) | i5 barcode | SampleID | Full barcode | Number | LiveOrDead | Notes |
AR | 150618 | Ch03 | A01 | N701 | S502 | TAAGGCGA | CTCTCTAT | AR-150618-Ch03-A01-N701-S502 | TAAGGCGA-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch02 | A02 | N702 | S502 | CGTACTAG | CTCTCTAT | AR-150618-Ch02-A02-N702-S502 | CGTACTAG-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch01 | A03 | N703 | S502 | AGGCAGAA | CTCTCTAT | AR-150618-Ch01-A03-N703-S502 | AGGCAGAA-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch49 | A04 | N704 | S502 | TCCTGAGC | CTCTCTAT | AR-150618-Ch49-A04-N704-S502 | TCCTGAGC-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch50 | A05 | N705 | S502 | GGACTCCT | CTCTCTAT | AR-150618-Ch50-A05-N705-S502 | GGACTCCT-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch51 | A06 | N706 | S502 | TAGGCATG | CTCTCTAT | AR-150618-Ch51-A06-N706-S502 | TAGGCATG-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch06 | A07 | N707 | S502 | CTCTCTAC | CTCTCTAT | AR-150618-Ch06-A07-N707-S502 | CTCTCTAC-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch05 | A08 | N710 | S502 | CGAGGCTG | CTCTCTAT | AR-150618-Ch05-A08-N710-S502 | CGAGGCTG-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch04 | A09 | N711 | S502 | AAGAGGCA | CTCTCTAT | AR-150618-Ch04-A09-N711-S502 | AAGAGGCA-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch52 | A10 | N712 | S502 | GTAGAGGA | CTCTCTAT | AR-150618-Ch52-A10-N712-S502 | GTAGAGGA-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch53 | A11 | N714 | S502 | GCTCATGA | CTCTCTAT | AR-150618-Ch53-A11-N714-S502 | GCTCATGA-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch54 | A12 | N715 | S502 | ATCTCAGG | CTCTCTAT | AR-150618-Ch54-A12-N715-S502 | ATCTCAGG-CTCTCTAT | 0 | na | na |
AR | 150618 | Ch09 | B01 | N701 | S503 | TAAGGCGA | TATCCTCT | AR-150618-Ch09-B01-N701-S503 | TAAGGCGA-TATCCTCT | 0 | na | na |
AR | 150618 | Ch08 | B02 | N702 | S503 | CGTACTAG | TATCCTCT | AR-150618-Ch08-B02-N702-S503 | CGTACTAG-TATCCTCT | 0 | na | na |
AR | 150618 | Ch07 | B03 | N703 | S503 | AGGCAGAA | TATCCTCT | AR-150618-Ch07-B03-N703-S503 | AGGCAGAA-TATCCTCT | 1 | dead | na |
AR | 150618 | Ch55 | B04 | N704 | S503 | TCCTGAGC | TATCCTCT | AR-150618-Ch55-B04-N704-S503 | TCCTGAGC-TATCCTCT | 0 | na | na |
AR | 150618 | Ch56 | B05 | N705 | S503 | GGACTCCT | TATCCTCT | AR-150618-Ch56-B05-N705-S503 | GGACTCCT-TATCCTCT | 0 | na | na |
AR | 150618 | Ch57 | B06 | N706 | S503 | TAGGCATG | TATCCTCT | AR-150618-Ch57-B06-N706-S503 | TAGGCATG-TATCCTCT | 0 | na | na |
AR | 150618 | Ch12 | B07 | N707 | S503 | CTCTCTAC | TATCCTCT | AR-150618-Ch12-B07-N707-S503 | CTCTCTAC-TATCCTCT | 1 | dead | na |
AR | 150618 | Ch11 | B08 | N710 | S503 | CGAGGCTG | TATCCTCT | AR-150618-Ch11-B08-N710-S503 | CGAGGCTG-TATCCTCT | 3 | dead | na |
AR | 150618 | Ch10 | B09 | N711 | S503 | AAGAGGCA | TATCCTCT | AR-150618-Ch10-B09-N711-S503 | AAGAGGCA-TATCCTCT | 1 | dead | weak |
AR | 150618 | Ch58 | B10 | N712 | S503 | GTAGAGGA | TATCCTCT | AR-150618-Ch58-B10-N712-S503 | GTAGAGGA-TATCCTCT | 0 | na | na |
AR | 150618 | Ch59 | B11 | N714 | S503 | GCTCATGA | TATCCTCT | AR-150618-Ch59-B11-N714-S503 | GCTCATGA-TATCCTCT | 0 | na | na |
AR | 150618 | Ch60 | B12 | N715 | S503 | ATCTCAGG | TATCCTCT | AR-150618-Ch60-B12-N715-S503 | ATCTCAGG-TATCCTCT | 0 | na | na |
AR | 150618 | Ch15 | C01 | N701 | S505 | TAAGGCGA | GTAAGGAG | AR-150618-Ch15-C01-N701-S505 | TAAGGCGA-GTAAGGAG | 2 | dead | na |
AR | 150618 | Ch14 | C02 | N702 | S505 | CGTACTAG | GTAAGGAG | AR-150618-Ch14-C02-N702-S505 | CGTACTAG-GTAAGGAG | 1 | dead | lumpy |
AR | 150618 | Ch13 | C03 | N703 | S505 | AGGCAGAA | GTAAGGAG | AR-150618-Ch13-C03-N703-S505 | AGGCAGAA-GTAAGGAG | 1 | live | extravasating |
AR | 150618 | Ch61 | C04 | N704 | S505 | TCCTGAGC | GTAAGGAG | AR-150618-Ch61-C04-N704-S505 | TCCTGAGC-GTAAGGAG | 1 | dead | weak |
AR | 150618 | Ch62 | C05 | N705 | S505 | GGACTCCT | GTAAGGAG | AR-150618-Ch62-C05-N705-S505 | GGACTCCT-GTAAGGAG | 0 | na | na |
AR | 150618 | Ch63 | C06 | N706 | S505 | TAGGCATG | GTAAGGAG | AR-150618-Ch63-C06-N706-S505 | TAGGCATG-GTAAGGAG | 0 | na | na |
AR | 150618 | Ch18 | C07 | N707 | S505 | CTCTCTAC | GTAAGGAG | AR-150618-Ch18-C07-N707-S505 | CTCTCTAC-GTAAGGAG | 1 | both | extravasating |
AR | 150618 | Ch17 | C08 | N710 | S505 | CGAGGCTG | GTAAGGAG | AR-150618-Ch17-C08-N710-S505 | CGAGGCTG-GTAAGGAG | 1 | live | extravasating |
AR | 150618 | Ch16 | C09 | N711 | S505 | AAGAGGCA | GTAAGGAG | AR-150618-Ch16-C09-N711-S505 | AAGAGGCA-GTAAGGAG | 1 | both | na |
AR | 150618 | Ch64 | C10 | N712 | S505 | GTAGAGGA | GTAAGGAG | AR-150618-Ch64-C10-N712-S505 | GTAGAGGA-GTAAGGAG | 0 | na | na |
AR | 150618 | Ch65 | C11 | N714 | S505 | GCTCATGA | GTAAGGAG | AR-150618-Ch65-C11-N714-S505 | GCTCATGA-GTAAGGAG | 0 | na | na |
AR | 150618 | Ch66 | C12 | N715 | S505 | ATCTCAGG | GTAAGGAG | AR-150618-Ch66-C12-N715-S505 | ATCTCAGG-GTAAGGAG | 0 | na | na |
AR | 150618 | Ch21 | D01 | N701 | S506 | TAAGGCGA | ACTGCATA | AR-150618-Ch21-D01-N701-S506 | TAAGGCGA-ACTGCATA | 1 | live | extravasating |
AR | 150618 | Ch20 | D02 | N702 | S506 | CGTACTAG | ACTGCATA | AR-150618-Ch20-D02-N702-S506 | CGTACTAG-ACTGCATA | 1 | dead | na |
AR | 150618 | Ch19 | D03 | N703 | S506 | AGGCAGAA | ACTGCATA | AR-150618-Ch19-D03-N703-S506 | AGGCAGAA-ACTGCATA | 0 | na | na |
AR | 150618 | Ch67 | D04 | N704 | S506 | TCCTGAGC | ACTGCATA | AR-150618-Ch67-D04-N704-S506 | TCCTGAGC-ACTGCATA | 0 | na | na |
AR | 150618 | Ch68 | D05 | N705 | S506 | GGACTCCT | ACTGCATA | AR-150618-Ch68-D05-N705-S506 | GGACTCCT-ACTGCATA | 0 | na | na |
AR | 150618 | Ch69 | D06 | N706 | S506 | TAGGCATG | ACTGCATA | AR-150618-Ch69-D06-N706-S506 | TAGGCATG-ACTGCATA | 0 | na | na |
AR | 150618 | Ch24 | D07 | N707 | S506 | CTCTCTAC | ACTGCATA | AR-150618-Ch24-D07-N707-S506 | CTCTCTAC-ACTGCATA | 1 | dead | extravasating |
AR | 150618 | Ch23 | D08 | N710 | S506 | CGAGGCTG | ACTGCATA | AR-150618-Ch23-D08-N710-S506 | CGAGGCTG-ACTGCATA | 1 | dead | na |
AR | 150618 | Ch22 | D09 | N711 | S506 | AAGAGGCA | ACTGCATA | AR-150618-Ch22-D09-N711-S506 | AAGAGGCA-ACTGCATA | 1 | live | extravasating |
AR | 150618 | Ch70 | D10 | N712 | S506 | GTAGAGGA | ACTGCATA | AR-150618-Ch70-D10-N712-S506 | GTAGAGGA-ACTGCATA | 1 | dead | extravasating |
AR | 150618 | Ch71 | D11 | N714 | S506 | GCTCATGA | ACTGCATA | AR-150618-Ch71-D11-N714-S506 | GCTCATGA-ACTGCATA | 0 | na | na |
AR | 150618 | Ch72 | D12 | N715 | S506 | ATCTCAGG | ACTGCATA | AR-150618-Ch72-D12-N715-S506 | ATCTCAGG-ACTGCATA | 0 | na | na |
AR | 150618 | Ch25 | E01 | N701 | S507 | TAAGGCGA | AAGGAGTA | AR-150618-Ch25-E01-N701-S507 | TAAGGCGA-AAGGAGTA | 1 | live | weak |
AR | 150618 | Ch26 | E02 | N702 | S507 | CGTACTAG | AAGGAGTA | AR-150618-Ch26-E02-N702-S507 | CGTACTAG-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch27 | E03 | N703 | S507 | AGGCAGAA | AAGGAGTA | AR-150618-Ch27-E03-N703-S507 | AGGCAGAA-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch75 | E04 | N704 | S507 | TCCTGAGC | AAGGAGTA | AR-150618-Ch75-E04-N704-S507 | TCCTGAGC-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch74 | E05 | N705 | S507 | GGACTCCT | AAGGAGTA | AR-150618-Ch74-E05-N705-S507 | GGACTCCT-AAGGAGTA | 1 | live | extravasating |
AR | 150618 | Ch73 | E06 | N706 | S507 | TAGGCATG | AAGGAGTA | AR-150618-Ch73-E06-N706-S507 | TAGGCATG-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch28 | E07 | N707 | S507 | CTCTCTAC | AAGGAGTA | AR-150618-Ch28-E07-N707-S507 | CTCTCTAC-AAGGAGTA | 1 | live | weak |
AR | 150618 | Ch29 | E08 | N710 | S507 | CGAGGCTG | AAGGAGTA | AR-150618-Ch29-E08-N710-S507 | CGAGGCTG-AAGGAGTA | 1 | live | extravasating |
AR | 150618 | Ch30 | E09 | N711 | S507 | AAGAGGCA | AAGGAGTA | AR-150618-Ch30-E09-N711-S507 | AAGAGGCA-AAGGAGTA | 1 | live | weak |
AR | 150618 | Ch78 | E10 | N712 | S507 | GTAGAGGA | AAGGAGTA | AR-150618-Ch78-E10-N712-S507 | GTAGAGGA-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch77 | E11 | N714 | S507 | GCTCATGA | AAGGAGTA | AR-150618-Ch77-E11-N714-S507 | GCTCATGA-AAGGAGTA | 1 | dead | weak |
AR | 150618 | Ch76 | E12 | N715 | S507 | ATCTCAGG | AAGGAGTA | AR-150618-Ch76-E12-N715-S507 | ATCTCAGG-AAGGAGTA | 0 | na | na |
AR | 150618 | Ch31 | F01 | N701 | S508 | TAAGGCGA | CTAAGCCT | AR-150618-Ch31-F01-N701-S508 | TAAGGCGA-CTAAGCCT | 2 | dead | na |
AR | 150618 | Ch32 | F02 | N702 | S508 | CGTACTAG | CTAAGCCT | AR-150618-Ch32-F02-N702-S508 | CGTACTAG-CTAAGCCT | 0 | na | na |
AR | 150618 | Ch33 | F03 | N703 | S508 | AGGCAGAA | CTAAGCCT | AR-150618-Ch33-F03-N703-S508 | AGGCAGAA-CTAAGCCT | 1 | dead | na |
AR | 150618 | Ch81 | F04 | N704 | S508 | TCCTGAGC | CTAAGCCT | AR-150618-Ch81-F04-N704-S508 | TCCTGAGC-CTAAGCCT | 0 | na | na |
AR | 150618 | Ch80 | F05 | N705 | S508 | GGACTCCT | CTAAGCCT | AR-150618-Ch80-F05-N705-S508 | GGACTCCT-CTAAGCCT | 1 | dead | na |
AR | 150618 | Ch79 | F06 | N706 | S508 | TAGGCATG | CTAAGCCT | AR-150618-Ch79-F06-N706-S508 | TAGGCATG-CTAAGCCT | 1 | dead | extravasating |
AR | 150618 | Ch34 | F07 | N707 | S508 | CTCTCTAC | CTAAGCCT | AR-150618-Ch34-F07-N707-S508 | CTCTCTAC-CTAAGCCT | 1 | live | weak |
AR | 150618 | Ch35 | F08 | N710 | S508 | CGAGGCTG | CTAAGCCT | AR-150618-Ch35-F08-N710-S508 | CGAGGCTG-CTAAGCCT | 2 | live | na |
AR | 150618 | Ch36 | F09 | N711 | S508 | AAGAGGCA | CTAAGCCT | AR-150618-Ch36-F09-N711-S508 | AAGAGGCA-CTAAGCCT | 0 | na | na |
AR | 150618 | Ch84 | F10 | N712 | S508 | GTAGAGGA | CTAAGCCT | AR-150618-Ch84-F10-N712-S508 | GTAGAGGA-CTAAGCCT | 0 | na | na |
AR | 150618 | Ch83 | F11 | N714 | S508 | GCTCATGA | CTAAGCCT | AR-150618-Ch83-F11-N714-S508 | GCTCATGA-CTAAGCCT | 0 | na | na |
AR | 150618 | Ch82 | F12 | N715 | S508 | ATCTCAGG | CTAAGCCT | AR-150618-Ch82-F12-N715-S508 | ATCTCAGG-CTAAGCCT | 1 | live | extravasating |
AR | 150618 | Ch37 | G01 | N701 | S510 | TAAGGCGA | CGTCTAAT | AR-150618-Ch37-G01-N701-S510 | TAAGGCGA-CGTCTAAT | 0 | na | na |
AR | 150618 | Ch38 | G02 | N702 | S510 | CGTACTAG | CGTCTAAT | AR-150618-Ch38-G02-N702-S510 | CGTACTAG-CGTCTAAT | 0 | dead | na |
AR | 150618 | Ch39 | G03 | N703 | S510 | AGGCAGAA | CGTCTAAT | AR-150618-Ch39-G03-N703-S510 | AGGCAGAA-CGTCTAAT | 1 | live | na |
AR | 150618 | Ch87 | G04 | N704 | S510 | TCCTGAGC | CGTCTAAT | AR-150618-Ch87-G04-N704-S510 | TCCTGAGC-CGTCTAAT | 0 | na | na |
AR | 150618 | Ch86 | G05 | N705 | S510 | GGACTCCT | CGTCTAAT | AR-150618-Ch86-G05-N705-S510 | GGACTCCT-CGTCTAAT | 1 | dead | na |
AR | 150618 | Ch85 | G06 | N706 | S510 | TAGGCATG | CGTCTAAT | AR-150618-Ch85-G06-N706-S510 | TAGGCATG-CGTCTAAT | 0 | na | na |
AR | 150618 | Ch40 | G07 | N707 | S510 | CTCTCTAC | CGTCTAAT | AR-150618-Ch40-G07-N707-S510 | CTCTCTAC-CGTCTAAT | 3 | dead | na |
AR | 150618 | Ch41 | G08 | N710 | S510 | CGAGGCTG | CGTCTAAT | AR-150618-Ch41-G08-N710-S510 | CGAGGCTG-CGTCTAAT | 1 | dead | na |
AR | 150618 | Ch42 | G09 | N711 | S510 | AAGAGGCA | CGTCTAAT | AR-150618-Ch42-G09-N711-S510 | AAGAGGCA-CGTCTAAT | 1 | dead | extravasating |
AR | 150618 | Ch90 | G10 | N712 | S510 | GTAGAGGA | CGTCTAAT | AR-150618-Ch90-G10-N712-S510 | GTAGAGGA-CGTCTAAT | 2 | both | na |
AR | 150618 | Ch89 | G11 | N714 | S510 | GCTCATGA | CGTCTAAT | AR-150618-Ch89-G11-N714-S510 | GCTCATGA-CGTCTAAT | 1 | both | weak |
AR | 150618 | Ch88 | G12 | N715 | S510 | ATCTCAGG | CGTCTAAT | AR-150618-Ch88-G12-N715-S510 | ATCTCAGG-CGTCTAAT | 1 | dead | na |
AR | 150618 | Ch43 | H01 | N701 | S511 | TAAGGCGA | TCTCTCCG | AR-150618-Ch43-H01-N701-S511 | TAAGGCGA-TCTCTCCG | 1 | dead | extravasating |
AR | 150618 | Ch44 | H02 | N702 | S511 | CGTACTAG | TCTCTCCG | AR-150618-Ch44-H02-N702-S511 | CGTACTAG-TCTCTCCG | 0 | na | debris |
AR | 150618 | Ch45 | H03 | N703 | S511 | AGGCAGAA | TCTCTCCG | AR-150618-Ch45-H03-N703-S511 | AGGCAGAA-TCTCTCCG | 1 | live | extravasating |
AR | 150618 | Ch93 | H04 | N704 | S511 | TCCTGAGC | TCTCTCCG | AR-150618-Ch93-H04-N704-S511 | TCCTGAGC-TCTCTCCG | 0 | na | na |
AR | 150618 | Ch92 | H05 | N705 | S511 | GGACTCCT | TCTCTCCG | AR-150618-Ch92-H05-N705-S511 | GGACTCCT-TCTCTCCG | 1 | live | bright |
AR | 150618 | Ch91 | H06 | N706 | S511 | TAGGCATG | TCTCTCCG | AR-150618-Ch91-H06-N706-S511 | TAGGCATG-TCTCTCCG | 0 | both | debris |
AR | 150618 | Ch46 | H07 | N707 | S511 | CTCTCTAC | TCTCTCCG | AR-150618-Ch46-H07-N707-S511 | CTCTCTAC-TCTCTCCG | 1 | dead | na |
AR | 150618 | Ch47 | H08 | N710 | S511 | CGAGGCTG | TCTCTCCG | AR-150618-Ch47-H08-N710-S511 | CGAGGCTG-TCTCTCCG | 1 | both | extravasating |
AR | 150618 | Ch48 | H09 | N711 | S511 | AAGAGGCA | TCTCTCCG | AR-150618-Ch48-H09-N711-S511 | AAGAGGCA-TCTCTCCG | 0 | na | na |
AR | 150618 | Ch96 | H10 | N712 | S511 | GTAGAGGA | TCTCTCCG | AR-150618-Ch96-H10-N712-S511 | GTAGAGGA-TCTCTCCG | 1 | live | extravasating |
AR | 150618 | Ch95 | H11 | N714 | S511 | GCTCATGA | TCTCTCCG | AR-150618-Ch95-H11-N714-S511 | GCTCATGA-TCTCTCCG | 1 | live | extravasating |
AR | 150618 | Ch94 | H12 | N715 | S511 | ATCTCAGG | TCTCTCCG | AR-150618-Ch94-H12-N715-S511 | ATCTCAGG-TCTCTCCG | 1 | dead | na |
Nextera PCR[edit]
Ran Nextera PCR on Eppendorf qPCR machine
Size selection[edit]
Sequencing[edit]
Hasty Lab Miseq | ' | ' | ' | ' | ' | ' | ' | ' | ' |
Kit Provider: Kun Zhang Lab | |||||||||
6/24/2015 | |||||||||
Loading Concentration: 26.5 pM | |||||||||
Sample ID | Index Sequence | # of Reads | Run Type, Minmum BP | Library Size | Gel Image | Read 1 Sequencing Primer | Index Primer | Read 2 Sequencing Primer | Notes |
INITIAL-PROJECT-SAMPLE-DATE-Index | CGTGCA | Million | SE/PE, 36+6+36 | SIZE RANGE (BP) | Link | Name | Name | Name | Labeling, pooling, quantification, etc |
AR-150618-Ch03-A01-N701-S502 | TAAGGCGA-CTCTCTAT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch02-A02-N702-S502 | CGTACTAG-CTCTCTAT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch07-B03-N703-S503 | AGGCAGAA-TATCCTCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch12-B07-N707-S503 | CTCTCTAC-TATCCTCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch13-C03-N703-S505 | AGGCAGAA-GTAAGGAG | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch17-C08-N710-S505 | CGAGGCTG-GTAAGGAG | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch21-D01-N701-S506 | TAAGGCGA-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch20-D02-N702-S506 | CGTACTAG-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch24-D07-N707-S506 | CTCTCTAC-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch23-D08-N710-S506 | CGAGGCTG-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch22-D09-N711-S506 | AAGAGGCA-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch70-D10-N712-S506 | GTAGAGGA-ACTGCATA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch74-E05-N705-S507 | GGACTCCT-AAGGAGTA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch29-E08-N710-S507 | CGAGGCTG-AAGGAGTA | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch33-F03-N703-S508 | AGGCAGAA-CTAAGCCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch80-F05-N705-S508 | GGACTCCT-CTAAGCCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch79-F06-N706-S508 | TAGGCATG-CTAAGCCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch82-F12-N715-S508 | ATCTCAGG-CTAAGCCT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch39-G03-N703-S510 | AGGCAGAA-CGTCTAAT | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
AR-150618-Ch45-H03-N703-S511 | AGGCAGAA-TCTCTCCG | 1 | SE50+8+8 | 250-800 | http://genome-tech.ucsd.edu/LabNotes/index.php/File:AR150618_C1_gels.png | HP10 | HP12 | NA | Tube labeled AR 6/18 |
Screening[edit]
File:AR150618 libraryComplexities.jpg
CNV profiling[edit]
File:AR-150618-Ch02-A02-N702-S502.all.png File:AR-150618-Ch03-A01-N701-S502.all.png File:AR-150618-Ch07-B03-N703-S503.all.png File:AR-150618-Ch12-B07-N707-S503.all.png File:AR-150618-Ch13-C03-N703-S505.all.png File:AR-150618-Ch17-C08-N710-S505.all.png File:AR-150618-Ch20-D02-N702-S506.all.png File:AR-150618-Ch21-D01-N701-S506.all.png File:AR-150618-Ch22-D09-N711-S506.all.png File:AR-150618-Ch23-D08-N710-S506.all.png File:AR-150618-Ch24-D07-N707-S506.all.png File:AR-150618-Ch29-E08-N710-S507.all.png File:AR-150618-Ch33-F03-N703-S508.all.png File:AR-150618-Ch39-G03-N703-S510.all.png File:AR-150618-Ch45-H03-N703-S511.all.png File:AR-150618-Ch70-D10-N712-S506.all.png File:AR-150618-Ch74-E05-N705-S507.all.png File:AR-150618-Ch79-F06-N706-S508.all.png File:AR-150618-Ch80-F05-N705-S508.all.png File:AR-150618-Ch82-F12-N715-S508.all.png
Lorenz Curves[edit]
File:AR-150618-Ch02-A02-N702-S502 lorenz.png File:AR-150618-Ch03-A01-N701-S502 lorenz.png File:AR-150618-Ch07-B03-N703-S503 lorenz.png File:AR-150618-Ch12-B07-N707-S503 lorenz.png File:AR-150618-Ch13-C03-N703-S505 lorenz.png File:AR-150618-Ch17-C08-N710-S505 lorenz.png File:AR-150618-Ch20-D02-N702-S506 lorenz.png File:AR-150618-Ch21-D01-N701-S506 lorenz.png File:AR-150618-Ch22-D09-N711-S506 lorenz.png File:AR-150618-Ch23-D08-N710-S506 lorenz.png File:AR-150618-Ch24-D07-N707-S506 lorenz.png File:AR-150618-Ch29-E08-N710-S507 lorenz.png File:AR-150618-Ch33-F03-N703-S508 lorenz.png File:AR-150618-Ch39-G03-N703-S510 lorenz.png File:AR-150618-Ch45-H03-N703-S511 lorenz.png File:AR-150618-Ch70-D10-N712-S506 lorenz.png File:AR-150618-Ch74-E05-N705-S507 lorenz.png File:AR-150618-Ch79-F06-N706-S508 lorenz.png File:AR-150618-Ch80-F05-N705-S508 lorenz.png File:AR-150618-Ch82-F12-N715-S508 lorenz.png
Mouse pipeline[edit]
Column purified MEF gDNA was used as a cell-free control. It was run through an identical pipeline constructed for mm10 instead of hg38
File:AR150618 mouse libraryComplexities.jpg
File:AR-150618-Ch02-A02-N702-S502 mouse.jpg File:AR-150618-Ch03-A01-N701-S502 mouse.jpg File:AR-150618-Ch07-B03-N703-S503 mouse.jpg File:AR-150618-Ch12-B07-N707-S503 mouse.jpg File:AR-150618-Ch13-C03-N703-S505 mouse.jpg File:AR-150618-Ch17-C08-N710-S505 mouse.jpg File:AR-150618-Ch20-D02-N702-S506 mouse.jpg File:AR-150618-Ch21-D01-N701-S506 mouse.jpg File:AR-150618-Ch22-D09-N711-S506 mouse.jpg File:AR-150618-Ch23-D08-N710-S506 mouse.jpg File:AR-150618-Ch24-D07-N707-S506 mouse.jpg File:AR-150618-Ch29-E08-N710-S507 mouse.jpg File:AR-150618-Ch33-F03-N703-S508 mouse.jpg File:AR-150618-Ch39-G03-N703-S510 mouse.jpg File:AR-150618-Ch45-H03-N703-S511 mouse.jpg File:AR-150618-Ch70-D10-N712-S506 mouse.jpg File:AR-150618-Ch74-E05-N705-S507 mouse.jpg File:AR-150618-Ch79-F06-N706-S508 mouse.jpg File:AR-150618-Ch80-F05-N705-S508 mouse.jpg File:AR-150618-Ch82-F12-N715-S508 mouse.jpg