Arichard:Notebook/midas150130
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January 1, 2015[edit]
MIDAS on 1-02 (AD) and 25-01 (CTL)
Gels[edit]
Used old 1:50 Nextera on first strip File:Midas150130Slide1.jpg File:Midas150130Slide2.jpg
I then tried 1X Tn5059 on strip 2 File:Midas150130Slide3.jpg File:Midas150130Slide4.jpg
Not sure why Tn5059 is not working on these MIDAS amplicons. It may have just been an unlucky batch. I repeated with the remaining samples.
File:Midas150130Slide5.jpg File:Midas150130Slide6.jpg File:Midas150130Slide7.jpg File:Midas150130Slide8.jpg
It looked like these ones worked, so I had Qiming go ahead with bead purification.
File:Midas150130Slide9.jpg File:Midas150130Slide10.jpg File:Midas150130Slide11.jpg File:Midas150130Slide12.jpg
Sequencing[edit]
Submitted:
Array/Sample # | Patient | NeuN | Tissue | PI peak | Sort date |
A2 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
A3 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
A4 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C19 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C20 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C21 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
J33 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
J35 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
K41 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
K44 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |