Arichard:Notebook/midas150130

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January 1, 2015[edit]

MIDAS on 1-02 (AD) and 25-01 (CTL)

Gels[edit]

Used old 1:50 Nextera on first strip File:Midas150130Slide1.jpg File:Midas150130Slide2.jpg

I then tried 1X Tn5059 on strip 2 File:Midas150130Slide3.jpg File:Midas150130Slide4.jpg

Not sure why Tn5059 is not working on these MIDAS amplicons. It may have just been an unlucky batch. I repeated with the remaining samples.

File:Midas150130Slide5.jpg File:Midas150130Slide6.jpg File:Midas150130Slide7.jpg File:Midas150130Slide8.jpg

It looked like these ones worked, so I had Qiming go ahead with bead purification.

File:Midas150130Slide9.jpg File:Midas150130Slide10.jpg File:Midas150130Slide11.jpg File:Midas150130Slide12.jpg

Sequencing[edit]

Submitted:

Array/Sample # Patient NeuN Tissue PI peak Sort date
A2 1-02 + Cortex Full 2N 9-Oct-14
A3 1-02 + Cortex Full 2N 9-Oct-14
A4 1-02 + Cortex Full 2N 9-Oct-14
C19 1-02 + Cortex Full 2N 9-Oct-14
C20 1-02 + Cortex Full 2N 9-Oct-14
C21 1-02 + Cortex Full 2N 9-Oct-14
J33 25-01 + Cortex Full 2N 9-Oct-14
J35 25-01 + Cortex Full 2N 9-Oct-14
K41 25-01 + Cortex Full 2N 9-Oct-14
K44 25-01 + Cortex Full 2N 9-Oct-14