Arichard:Reagents/als
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ALS (Alkaline lysis solution) shelf life is two weeks.
To make ALS:
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA.
KOH shelf life is also two weeks.
Alternatively:
- 90 ul 0.11 M DTT
- 8 ul 5 M KOH
- 2 ul 0.5 M EDTA