Arichard:Reagents/als

From ZhangLabWiki
Jump to navigation Jump to search

ALS (Alkaline lysis solution) shelf life is two weeks.

To make ALS:

  • 80 ul Nuclease free H2O.
  • 8 ul 5 M KOH.
  • 10 ul 1 M DTT.
  • 2 ul 0.5 M EDTA.

KOH shelf life is also two weeks.

Alternatively:

  • 90 ul 0.11 M DTT
  • 8 ul 5 M KOH
  • 2 ul 0.5 M EDTA