Arichard:Reagents/hmw buffer

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Email forwarded from Jeff:


From the original patents of Epicentre and Reznikoffs publications, we think the HMW buffer contain the following:

Reaction Buffer:      (1x Illumina Bf = pH 7.27 @RT, density=10% DMF) 
5X TA buffer: 165 mM Tris-OAc, pH 7.8, 50 mM Mg-OAc, 330 mM K-OAc, 50% DMF.


From experimenting with the buffer, we obtain longer fragments when lowering the DMF. This is likely due to the DMF favors the bending of the DNA to enable the looping of shorter fragments. I hope this is of help.


End of email.