Arichard:Reagents/nexteraDilution

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UV treat TE and glycerol before adding enzyme.

From Shendure protocol in Jeff's notes: 1 μl Nextera enzyme + 24 μl TE + 25 μl 100% glycerol.

It is easier, however, to make a large solution of TE/glycerol to avoid pipeting small volumes of glycerol.

For example, mix 2.4 ml TE + 2.5 ml 100% glycerol --> 49 ul of this solution + 1 ul of Nextera = 50 ul 1:50 dilution.