Arichard:Samples/MDA microarray samples

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MDA microarray[edit]

September 13[edit]

  • All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum).
    • 2401 CTX+ needs no dilution.
    • 2401 CBL+ needs 1:10 dilution.
    • Jeff didn't say what dilutions are needed for 2401 CTX- and 2401 CBL-.
  • Other samples are as follows
    • Two types of sea bacteria: Synechococcus and prochlorococcus. These samples are already at the right concentrations, 10-20 parts/ul.
    • Skin bacteria sample from microbiome project. Heterogeneous. I need to ask Jeff about these dilutions.
    • Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
    • Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
  • Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.

October 10[edit]

  • Below is the current list of all the samples and required dilutions.
Samples and dilutions
Skn 1:1
Sea 1:1
CtlCtx+ 1:10
CtlCtx- 1:10
AlzCtx+ 1:1
AlzCtx- 1:10
AlzCbl+ 1:20
AlzCbl- 1:10
DowCtx+ 1:10
DowCtx 1:20

09/25/2013[edit]

Four new samples from Chun lab received last week:

  • Non-diseased, neuronal (1568 +)
  • Non-diseased, non-neuronal (1568 -)
  • Alzheimer's, neuronal (25-00 +)
  • Alzheimer's, non-neuronal (25-00 -)

According to Gwen: Fixed in ethanol, suspended in PBS.