Arichard:Samples/sort141021

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2014 October 21 sort for SW480 and lymphocytes[edit]

  • 6 tubes sorted sorted into 1% BSA PBSE (sat in tube at least 1 hr prior)
    • SW480 had trimodal PI distribution, suggesting N, 2N, and 4N populations
  • Spun down at 900 RCF for 10 min
  • Resuspended in 100 ul PBS with 1% BSA and 10% DMSO, counted on BioRad TC20
  • Aliquoted 10 tubes each with 10 ul/tube

Tubes (numbered)

  1. SW480 2N Hi PI, aliquoted @ 2.8e5/ml
  2. SW480 2N Lo PI, aliquoted @ 2.54e5/ml
  3. SW480 N Full PI, aliquoted @ 2.8e5/ml
  4. SW480 2N Full PI, aliquoted @ 4.3e5/ml
  5. SW480 4N Full PI, aliquoted @ 2.63e5/ml
  6. Lymphocytes, aliquoted @ 5.31e5/ml