Arichard:Samples/sort141021
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2014 October 21 sort for SW480 and lymphocytes[edit]
- 6 tubes sorted sorted into 1% BSA PBSE (sat in tube at least 1 hr prior)
- SW480 had trimodal PI distribution, suggesting N, 2N, and 4N populations
- Spun down at 900 RCF for 10 min
- Resuspended in 100 ul PBS with 1% BSA and 10% DMSO, counted on BioRad TC20
- Aliquoted 10 tubes each with 10 ul/tube
Tubes (numbered)
- SW480 2N Hi PI, aliquoted @ 2.8e5/ml
- SW480 2N Lo PI, aliquoted @ 2.54e5/ml
- SW480 N Full PI, aliquoted @ 2.8e5/ml
- SW480 2N Full PI, aliquoted @ 4.3e5/ml
- SW480 4N Full PI, aliquoted @ 2.63e5/ml
- Lymphocytes, aliquoted @ 5.31e5/ml